|
KMID : 0359319910310020217
|
|
Korean Journal of Veterinary Research
1991 Volume.31 No. 2 p.217 ~ p.222
|
|
|
A study on production of early pregnancy diagnostic kit in cattle
|
|
|
|
|
Abstract
|
|
|
Most progesterone enzyme immunoassays(EIA) are used liquid phase double-antibody separation. These methods consume considerable time and reagents because of the requirements for several washing and centrifugation steps involving the reactants.
Because of there several problems, we were prompted to develop an effective EIA system by the use of higher titer of progesterone antiserum free of anti-bovine serum albumin antibodies (anti-BSA).
The results obtained were as follows.
1. The antibody of progesterone antiserum was high as 1.5×10^5.
2. Percent activity bound of progesterone antiserum was about 77 at a dilution to 5×l0³ times.
3. Progesterone antiserum was contained a large amount of anti-BSA antibodies.
4. The anti-BSA was completely absorbed by using of polymerised BSA.
5. The molecular weight of albumin polymer (polymerised BSA) obtained by using 2.5% glutaraldehyde was 5×10^5.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|